研 究 業 績


 ここでは,NKKにおける最近の主な研究業績(著書,論文等)をまとめました.それぞれの論文等の詳細を知りたい方は,中野までご連絡ください.


組織培養に関する研究業績


Masaru Nakano, Yusuke Watanabe and Yoichiro Hoshino (2000) Histological examinations of callogenesis and adventitious embryogenesis in immature ovary culture of grapevine (Vitis vinifera L.). Journal of Horticultural Science and Biotechnology 75: 154−160

Masaru Nakano, Toshiaki Sakakibara, Sakae Suzuki and Hiroyuki Saito (2000) Decrease in the regeneration potential of long-term cell suspension cultures of
Lilium formosanum Wallace and its restoration by the auxin transport inhibitor, 2,3,5-triiodobenzoic acid. Plant Science 158: 129–137

Sakae Suzuki and Masaru Nakano (2001) Organogenesis and somatic embryogenesis from callus cultures in
Muscari armeniacum Leichtl. ex Bak. In Vitro Cellular & Developmental Biology - Plant 37: 382–387

Sakae Suzuki, Miki Oota and Masaru Nakano (2002) Embryogenic callus induction from leaf explants of the Liliaceous ornamental plant,
Agapanthus praecox ssp. orientalis (Leighton) Leighton. Histological study and response to selective agents. Scientia Horticulturae 95: 123–132

Hiroyuki Saito and Masaru Nakano (2002) Plant regeneration from suspension cultures of
Hosta sieboldiana (Lodd.) Engl. Plant Cell, Tissue and Organ Culture 71: 23–28

Sakae Suzuki and Masaru Nakano
(2003) Effects of antibiotics and bialaphos on the growth and development of embryogenic callus cultures of Muscari armeniacum. Biologia Plantarum 47: 425–427

Masaru Nakano, Keiko Mizunashi, Shigefumi Tanaka, Toshinari Godo, Masashi Nakata and Hiroyuki Saito (2004) Somatic embryogenesis and plant regeneration from callus cultures of several species in the genus
Tricyrtis. In Vitro Cellular & Developmental Biology - Plant 40: 274–278

Shiro Mori and Masaru Nakano (2004) Somatic embryo induction from leaf- and flower bud-derived calli in several
Muscari species and cultivars. Propagation of Ornamental Plants 4: 58–62

Masaru Nakano, Miho Nagai, Shigefumi Tanaka, Masashi Nakata and Toshinari Godo (2005) Adventitious shoot regeneration and micropropagation of the Japanese endangered
Hylotelephium sieboldii (Sweet ex Hook.) H. Ohba and H. sieboldii var. ettyuense (Tomida) H. Ohba. Plant Biotechnology 22: 221–224

Shiro Mori, Yukiko Adachi, Sakae Horimoto, Sakae Suzuki and Masaru Nakano (2005) Callus formation and plant regeneration in various
Lilium species and cultivars. In Vitro Cellular & Developmental Biology - Plant 41: 783–788

Masaru Nakano, Toshikazu Nomizu, Keiko Mizunashi, Mami Suzuki, Shiro Mori, Sachiko Kuwayama, Megumi Hayashi, Hiroto Umehara, Eriko Oka, Hiroyuki Kobayashi, Megumi Asano, Shintaro Sugawara, Hiroki Takagi, Hiroyuki Saito, Masashi Nakata, Toshinari Godo, Yoshihiro Hara and Junji Amano (2006) Somaclonal variation in
Tricyrtis hirta plants regenerated from 1-year-old embryogenic callus cultures. Scientia Horticulturae 110: 366–371

Masaru Nakano,
Hiroki Takagi, Shintaro Sugawara, Tomoka Saito, Yusuke Watanabe, Lu Yuanxue, Guan Kaiyun and Toshinari Godo (2009) Adventitious shoot regeneration and micropropagation of Chirita flavimaculata W. T. Wang, C. eburnea Hance, and C. speciosa Kurz. Propagation of Ornamental Plants 9: 216–222

Masaru Nakano
, Yusuke Watanabe, Toshikazu Nomizu, Mami Suzuki, Keiko Mizunashi, Shiro Mori, Junji Amano, Dong-Sheng Han, Hiroyuki Saito, Hiromichi Ryuto, Nobuhisa Fukunishi and Tomoko Abe (2010) Promotion of somatic embryo production from embryogenic calluses of monocotyledonous and dicotyledonous plants by heavy-ion beam irradiation. Plant Growth Regulation 60: 169–173

Masaru Nakano, Junji Amano
, Yusuke Watanabe, Toshikazu Nomizu, Mami Suzuki, Keiko Mizunashi, Shiro Mori, Sachiko Kuwayama, Dong-Sheng Han, Hiroyuki Saito, Hiromichi Ryuto, Nobuhisa Fukunishi and Tomoko Abe (2010) Morphological variation in Tricyrtis hirta plants regenerated from heavy-ion beam irradiated embryogenic calluses. Plant Biotechnology 27: 155–160

Hiroki Takagi, Shintaro Sugawara, Tomoka Saito, Haruka Tasaki, Lu Yuanxue, Guan Kaiyun, Dong-Sheng Han, Toshinari Godo and Masaru Nakano (2011) Plant regeneration via direct and indirect adventitious shoot formation and chromosome-doubled somaclonal variation in
Titanotrichum oldhamii (Hemsl.) Solereder. Plant Biotechnology Reports 5: 187−195

Takahiro Nonaka, Eriko Oka, Megumi Asano, Sachiko Kuwayama, Hiromu Tasaki, Dong-Sheng Han, Toshinari Godo and Masaru Nakano (2011) Chromosome doubling of
Lychnis spp. by in vitro spindle toxin treatment of nodal segments. Scientia Horticulturae 129: 832−839

Alexis K. Vidal, Dong-Sheng Han, Masaru Nakano and Yoshiji Niimi (2012) Decreased time from seed to flowering corm size in
Zephyra elegans via in vitro cultivation. Ciencia e Investigación Agraria 39: 577−584

Phithak Inthima, Masahiro Otani, Tomoya Hirano, Yoriko Hayashi, Tomoko Abe, Masaru Nakano and Kanyaratt Supaibulwatana (2014) Mutagenic effects of heavy-ion beam irradiation on in vitro nodal segments of
Artemisia annua L. Plant Cell, Tissue and Organ Culture 119: 131−139


プロトプラスト・細胞融合に関する研究業績


Hiroyuki Saito and Masaru Nakano (2001) Flow cytometric analysis of partially synchronized suspension cultures of Hemerocallis hybrida. Plant Biotechnology 18: 229–231

Hiroyuki Saito and Masaru Nakano (2001) Partial synchronization of cell division and micronucleation in suspension-cultured cells of
Hemerocallis hybrida: the effects of hydroxyurea and various spindle toxins. Breeding Science 51: 285–291

Hiroyuki Saito and Masaru Nakano (2002) Isolation and characterization of microprotoplasts from propyzamide-treated cell suspension cultures of
Hemerocallis hybrida. Breeding Science 52: 51–56

Hiroyuki Saito and Masaru Nakano (2002) Isolation and characterization of gametic microprotoplasts from developing microspores of
Lilium longiflorum for partial genome transfer in the Liliaceous ornamentals. Sexual Plant Reproduction 15: 179–185

Hiroyuki Saito and Masaru Nakano (2002) Preparation of microprotoplasts for partial genome transfer via microprotoplast fusion in Liliaceous ornamental plants.
Japan Agricultural Research Quarterly 36: 129–135

Masaru Nakano, Shigefumi Tanaka, Miki Oota, Eisuke Ookawa, Sakae Suzuki and Hiroyuki Saito (2003) Regeneration of diploid and tetraploid plants from callus-derived protoplasts of
Agapanthus praecox ssp. orientalis (Leighton) Leighton. Plant Cell, Tissue and Organ Culture 72: 63–69

Masaru Nakano, Shigefumi Tanaka, Shiho Kagami and Hiroyuki Saito (2005) Plantlet regeneration from protoplasts of
Muscari armeniacum Leichtl. ex Bak. Plant Biotechnology 22: 249–251


遠縁種間交雑に関する研究業績


Toru Nakamura, Sachiko Kuwayama, Shigefumi Tanaka, Tomo Oomiya, Hiroyuki Saito and Masaru Nakano (2005) Production of intergeneric hybrid plants between Sandersonia aurantiaca and Gloriosa rothschildiana via ovule culture (Colchicaceae). Euphytica 142: 283–289

Sachiko Kuwayama, Toru Nakamura, Yoko Mizuta, Tomo Oomiya and Masaru Nakano (2005) Cross-compatibility in interspecific and intergeneric hybridization among the Colchicaceous ornamentals,
Gloriosa spp., Littonia modesta and Sandersonia aurantiaca. Acta Horticulturae 673: 421–427

Junji Amano, Juntaro Kato, Masaru Nakano and Masahiro Mii (2006) Production of inter-section hybrids between
Primula filchnerae and P. sinensis through ovule culture. Scientia Horticulturae 110: 223–227

Junji Amano, Sachiko Kuwayama, Yoko Mizuta, Tomo Oomiya, Toru Nakamura and Masaru Nakano (2007) Early identification of intra- and intergeneric hybrids among Colchicaceous ornamentals,
Gloriosa spp., Littonia modesta Hook. and Sandersonia aurantiaca Hook., by flow cytometry and random amplified polymorphic DNA analyses. Journal of the Japanese Society for Horticultural Science 76: 73–78

Junji Amano, Sachiko Kuwayama, Yoko Mizuta, Toshinari Godo, Hajime Okuno and Masaru Nakano (2008) Morphological characterization of three intergeneric hybrids among
Gloriosa superba ‘Lutea’, Littonia modesta and Sandersonia aurantiaca (Colchicaceae). HortScience 43: 115–118

Masaru Nakano and Masahiro Mii (2008) Recent advence in wide hybridization and embryo rescue of floricultural plants in Japan.
Plant Biotechnology 25: 509

Toshinari Godo, Junko Miyazaki, Sachuiko Kuwayama, Shinjiro Ogita, Yasuo Kato, Masaru Nakano and Masashi Nakata (2009) Interspecific hybridization between triploid Senno (
Lychnis senno Siebold et Zucc., Caryophyllaceae) and allied taxa of the gennus Lychnis. Plant Biotechnology 26: 301–305

Junji Amano, Daisuke Nakazawa, Sachiko Kuwayama, Yoko Mizuta, Hajime Okuno, Yusuke Watanabe, Toshinari Godo, Dong-Sheng Han and Masaru Nakano (2009) Intergeneric hybridization among Colchicaceous ornamentals,
Gloriosa spp., Littonia modesta and Sandersonia aurantiaca via ovule culture. Plant Biotechnology 26: 535–541

Daisuke Nakazawa, Tomonari Kishimoto, Takeru Sato, Tomoka Saito, Junji Amano, Sachiko Kuwayama, Hajime Okuno, Toshinari Godo, Yusuke Watanabe, Dong-Sheng Han and Masaru Nakano (2011) Genomic
in situ hybridization (GISH) analysis of intergeneric hybrids in Colchicaceae. Euphytica 181: 197−202

Masaru Nakano, Sachiko Kuwayama, Eriko Oka, Megumi Asano, Dong-Sheng Han and Toshinari Godo (2013) Cross-compatibility in interspecific hybridization of
Lychnis (Caryophyllaceae) and characterization of interspecific hybrids between L. fulgens and L. sieboldii. Journal of the Japanese Society for Horticultural Science 82: 57−62

Haruka Tasaki, Hiroki Takagi, Hanae Otsubo, Chika Hatakeyama, Yusaku Saito, Eriko Oka, Junji Amano, Dong-Sheng Han and Masaru Nakano (2014) Interspecific hybridization between
Tricyrtis flava and T. formosana via ovule culture. Acta Horticulturae 1025: 43−50

Tomonari Kishimoto, Daisuke Nakazawa, Takeru Sato, Tomoka Saito, Junji Amano, Sachiko Kuwayama, Dong-Sheng Han and Masaru Nakano (2014) Horticultural characterization of an intergeneric hybrid between a septaploid
Gloriosa cultivar and Sandersonia aurantiaca. Acta Horticulturae 1025: 51−56

Tomonari Kishimoto, Miki Yamakawa, Daisuke Nakazawa, Junji Amano, Sachiko Kuwayama and Masaru Nakano (2014) Meiotic chromosome pairing in intergeneric hybrids of colchicaceous ornamentals revealed by genomic
in situ hybridization (GISH). Euphytica 200: 251−257


遺伝子組換えに関する研究業績


Sakae Suzuki, Kanyaratt Supaibulwatana, Masahiro Mii and Masaru Nakano (2001) Production of transgenic plants of the Liliaceous ornamental plant Agapanthus praecox ssp. orientalis (Leighton) Leighton via Agrobacterium-mediated transformation of embryogenic calli. Plant Science 161: 89–97

Sakae Suzuki and Masaru Nakano (2002)
Agrobacterium-mediated production of transgenic plants of Muscari armeniacum Leichtl. ex Bak. Plant Cell Reports 20: 835–841

Sakae Suzuki and Masaru Nakano (2002)
Agrobacterium-mediated transformation in Liliaceous ornamental plants. Japan Agricultural Research Quarterly 36: 119–127

Yosuke Koike, Yoichiro Hoshino, Masahiro Mii and Masaru Nakano (2003) Horticultural characterization of
Angelonia salicariifolia plants transformed with wild-type strains of Agrobacterium rhizogenes. Plant Cell Reports 21: 981–987

Yosuke Hoshi, Masayoshi Kondo, Shiro Mori, Yukiko Adachi, Masaru Nakano and Hitoshi Kobayashi (2004) Production of transgenic lily plants by
Agrobacterium-mediated transformation. Plant Cell Reports 22: 359–364

Shiro Mori, Hitoshi Kobayashi, Yosuke Hoshi, Masayoshi Kondo and Masaru Nakano (2004) Heterologous expression of the flavonoid 3’,5’-hydroxylase gene of
Vinca major alters flower color in transgenic Petunia hybrida. Plant Cell Reports 22: 415–421

Sakae Suzuki, Masaru Nakano, Yosuke Koike, Kenji Ueda, Masayasu Inoue, Masahiro Nishihara and Saburo Yamamura (2005) Comparison of selection efficiency between the bar and hpt genes in
Agrobacterium-mediated transformation of Muscari armeniacum. Journal of the Japanese Society for Horticultural Science 74: 60–62

Yukiko Adachi, Shiro Mori and Masaru Nakano (2005)
Agrobacterium-mediated production of transgenic plants in Tricyrtis hirta (Liliaceae). Acta Horticulturae 673: 415–419

Shiro Mori, Hitoshi Kobayashi, Yosuke Hoshi, Masayoshi Kondo and Masaru Nakano (2005) Isolation and characterization of a cytochrome P450 gene from
Muscari armeniacum. Acta Horticulturae 673: 429–435

Yosuke Hoshi, Masayoshi Kondo, Shiro Mori, Masaru Nakano and Hitoshi Kobayashi (2005)
Agrobacterium-mediated transformation of Lilium longiflorum. Acta Horticulturae 673: 543–547

Toru Nakamura, Tatsuya Fukuda, Masaru Nakano, Mitsuyasu Hasebe, Toshiaki Kameya and Akira Kanno (2005) The modified ABC model explains the development of the petaloid perianth of
Agapanthus praecox ssp. orientalis (Agapanthaceae) flowers. Plant Molecular Biology 58: 435–445

Masaru Nakano, Shiro Mori, Sakae Suzuki, Yosuke Hoshi and Hitoshi Kobayashi (2006) Production of transgenic plants via
Agrobacterium-mediated transformation in Liliaceous ornamentals. In: J. A. T. da Silva (ed.) Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, Volume II, Global Science Books, UK, pp. 172–183

Shiro Mori, Eriko Oka, Hiroto Umehara, Sakae Suzuki, Hitoshi Kobayashi, Yosuke Hoshi, Masayoshi Kondo, Yosuke Koike and Masaru Nakano (2007) Somaclonal variation and stability of GUS gene expression in transgenic agapanthus (
Agapanthus praecox ssp. orientalis) plants at the flowering stage. In Vitro Cellular & Developmental Biology – Plant 43: 79–87

Masaru Nakano, Hiroto Umehara, Yoshihiro Hara, Motohide Makino, Mika Igarashi, Mutsumi Nakada, Toru Nakamura, Yoichiro Hoshino and Akira Kanno (2007) Flower form alteration by genetic transformation with the class B MADS-box genes of
Agapanthus praecox spp. orientalis in transgenic dicot and monocot plants. Molecular Breeding 20: 425–429

Shiro Mori, Eriko Oka, Hiroto Umehara, Hitoshi Kobayashi, Yosuke Hoshi, Masayoshi Kondo, Yosuke Koike and Masaru Nakano (2008) Stability of GUS gene expression in transgenic
Tricyrtis hirta plants after two years of cultivation. Biologia Plantarum 52: 513−516

Yukiko Kamiishi, Masahiro Otani, Hiroki Takagi, Dong-Sheng Han, Shiro Mori, Fumi Tatsuzawa, Hiroaki Okuhara, Hitoshi Kobayashi and Masaru Nakano (2012) Flower color alteration in the liliaceous ornamental
Tricyrtis sp. by RNA interference-mediated suppression of the chalcone synthase gene. Molecular Breeding 30: 671−680

Masahiro Otani, Shuhei Meguro, Haruka Gondaira, Megumi Hayashi, Misaki Saito, Dong-Sheng Han, Phithak Inthima, Kanyaratt Supaibulwatana, Shiro Mori, Yusuke Jikumaru, Yuji Kamiya, Tuoping Li, Tomoya Niki, Takaaki Nishijima, Masaji Koshioka and Masaru Nakano (2013) Overexpression of the gibberellin 2-oxidase gene from
Torenia fournieri induces dwarf phenotypes in the liliaceous monocotyledon Tricyrtis sp. Journal of Plant Physiology 170: 1416−1423

Shiro Mori, Masahiro Otani, Hitoshi Kobayashi and Masaru Nakano (2014) Isolation and characterization of the dihydroflavonol 4-reductase gene in the monocotyledonous ornamental
Agapanthus praecox ssp. orientalis (Leighton) Leighton. Scientia Horticulturae 166: 24−30

Yoichiro Hoshino, Toshiya Igarashi, Masumi Narita, Koichi Shinoda, Naho Murata, Akira Kanno and Masaru Nakano (2014) Characterization of
CYCLOIDEA-like genes in controlling floral zygomorphy in the monocotyledon Alstroemeria. Scientia Horticulturae 169: 6−13

Masahiro Otani, Shuhei Meguro, Haruka Gondaira, Megumi Hayashi, Misaki Saito, Dong-Sheng Han and Masaru Nakano (2014) Overexpression of the gibberellin 20-oxidase or gibberellin 3-oxidase gene from
Torenia fournieri affects plant morphology in transgenic Tricyrtis sp. Acta Horticulturae 1025: 23−30

Nao Okuzumi, Masahiro Otani, Hanae Otsubo, Shuhei Meguro, Yoshihiro Hara, Hiroto Umehara, Mika Igarashi, Dong-Sheng Han and Masaru Nakano (2014) Characterization of transgenic
Agapanthus praecox ssp. orientalis plants ectopically expressing the class B MADS-box genes. Acta Horticulturae 1025: 93−98

Masahiro Otani, Mitsuyo Ishibe, Phithak Inthima, Kanyaratt Supaibulwatana, Shiro Mori, Tomoya Niki, Takaaki Nishijima, Masaji Koshioka and Masaru Nakano (2014) Horticultural characterization of a tetraploid transgenic plant of
Tricyrtis sp. carrying the gibberellin 2-oxidase gene from Torenia fournieri. Plant Biotechnology 31: 335−340

Ahmad Sharifi, Kanako Oizumi, Shosei Kubota, Abdolreza Bagheri, Saeid Malekzadeh Shafaroudi, Masaru Nakano and Akira Kanno (2015) Double flower formation in
Tricyrtis macranthopsis is related to low expression of AGAMOUS ortholog gene. Scientia Horticulturae 193: 337−345

Masahiro Otani, Ahmad Sharifi, Shosei Kubota, Kanako Oizumi, Fumi Uetake, Masayo Hirai, Yoichiro Hoshino, Akira Kanno and Masaru Nakano (2016) Suppression of B function strongly supports the modified ABCE model in
Tricyrtis sp. (Liliaceae). Scientific Reports 6: 24549, DOI: 10.1038/srep24549


その他


Yusuke Watanabe, Kazunori Miyajima, Toshikazu Nomizu, Masaru Nakano and Kazuo Ichimura (2013) Effect of sugar treatments on the verse life of cut tulip (Tulipa gesneriana L.) flowers. Horticultural Research (Japan) 12: 201−207

Naonori Hamato, Osamu Kotake, Nagaaki Ono, Hiroshi Kurashima, Masaru Nakano, Yuzuri Iwamoto and Yoshihiko Takahashi (2014) ‘Niigata S3’ is a new strawberry cultivar suitable for forcing culture under low temperature and insolation conditions.
Breeding Science 64: 427−434